بررسی همزیستی قارچ‎های مایکوریز آربوسکولار همراه با ریشه پنبه در استان خراسان شمالی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی‌ارشد، گروه گیاهپزشکی دانشکده کشاورزی دانشگاه زابل

2 استادیار گروه گیاهپزشکی دانشکده کشاورزی دانشگاه زابل

چکیده

به‌منظور بررسی وجود همزیستی قارچ­های مایکوریز با ریشه گیاه پنبه، تعیین درصد کلونیزاسیون ریشه­ها و مشخص کردن وضعیت اسپور این قارچ­ها، بیست و دو نمونه خاک به همراه ریشه از مزارع پنبه مناطق مختلف استان خراسان شمالی جمع­آوری گردید. پس از رنگ‌آمیزی ریشه­ها و استخراج اسپورها از خاک، میزان کلونیزاسیون ریشه و جمعیت اسپور این قارچ­ها اندازه‌گیری شد. همچنین تاثیر pH خاک بر این عوامل مورد بررسی قرار گرفت. نتایج بدست آمده نشان داد همزیستی مایکوریزی آربوسکولار همراه با ریشه پنبه وجود دارد. در مورد شاخص­های مرتبط با کلونیزاسیون میانگین فراوانی مایکوریزایی (%F) در مناطق مختلف از 95 تا 100 درصد برآورد شد. شاخص تراکم مایکوریزایی (%M) در مناطق سنخواست و آشخانه با میانگین‌های 8/59 و 9/31 به‌ترتیب بالاترین و پایین­ترین مقدار بود. در مورد شاخص تراکم جمعیت اسپوری بالاترین و پایین­ترین تراکم در مناطق اسفراین و آشخانه به‌ترتیب با میانگین 9/212 و 2/137 اسپور در 35 گرم خاک خشک بدست آمد. بررسی همبستگی شاخص‌های اندازه­گیری شده با pH خاک نشان داد که همبستگی شاخص تراکم اسپور با pH در سطح احتمال 05/0 معنی‌دار و از نوع مثبت است.

کلیدواژه‌ها


عنوان مقاله [English]

Survey of Arbuscular Mycorrhizal Fungi symbiosis with cotton root in north khorasan province

نویسندگان [English]

  • H. Shirzad 1
  • M. Ghorbany 2
1 1Former M.Sc. Student, Department of Plant Protection, College of Agriculture University of Zabol
2 Assistant Professor, Department of Plant Protection, College of Agriculture University of Zabol
چکیده [English]

To investigation existence of arbuscular mycorrhizal fungi symbiosis with cotton root, determination of root colonization percent and characterization of spore situation, 22 soil and root samples gathered from cotton fields of different areas in north Khorasan province. After root staining and extraction of spores from soil, root colonization and spore population determined, also effect of soil pH on these factors was evaluated. Results showed that all samples have arbuscular mycorrhizal symbiosis. About indices related to colonization, average of mycorrhizal frequency (%F) in different areas was between 95 to 100 percent. Average of mycorrhizal intensity index (%M) in Sankhast and Ashkhaneh areas with 59.8 and 31.9 was maximum and minimum respectively. about spore population density index maximum and minimum was in Esfarayen and Ashkhaneh with 212.9 and 137.2 spores per 35g/dried soil respectively. Correlation of measured indices with pH showed that correlation of spore population density with pH was significant (α = 0.05) and this correlation was positive.

کلیدواژه‌ها [English]

  • mycorrhiza
  • Arbuscular
  • North Khorasan
  • cotton
Anonymous. 2011. Agriculture marnamh. Office of statistics and information
technology, ministry of agriculture, Pp. 14-49. Tehran, Iran.
Bever, J.D., Schultz, P. A., Pringle, A., and Morton, J. B. 2001. Arbuscular mycorrhizal
fungi: more diverse than meets the age and the ecological tale of why. J.
Bioscience, 51:923-931.
Boddington, C.L., and Dodd, J.C. 2000. The effect of agricultural practices on the
development of indigenous arbuscular mycorrhizal fungi. I. Field studies in an
Indonesian. J. Plant and Soil, 218:137-144.
Bowen, G.D. 1980. Mycorrhizal Roled in Tropical Palnts and Ecosystems. In
Tropical Mycorrhizae research. Oxford university press. 19-165.
Brundrett, M. 1991. Mycorrhizas in natural ecosystem. J .Advance, Ecology.
Research. 21: 171-313.
Brundrett, M.C., Abbott, L.K., and Jasper, D.A. 1999. Glomalean fungi from
tropical Australia. I. Comparison of the effectiveness and specificity of different
isolation procedures. J. Mycorrhiza, 8:305–314.
Eom, A.H., David, C., Hartnett, A., Gail, W.T., and Wilson, C. 2000. Host plant
species effects on arbuscular mycorrhizal fungal communities in tallgrass
prairie. J. Oecologia, 122:435-444.
Esmaeilzadeh, S., Zare-maivan, H., and Ganatim, F. 2005. Vesicular arbuscular
mycorrhiza symbiosis in medicinal plants of Tandooreh national park. Iranian J.
Medicinal and Aromatic Plants. 21: 489-504.
Gay, P.E., Gyubb, P.J., and Hudson, H.J. 1982. Season changes in the
concentrations of Nitrogen, Phosphorus and Potassium, and in the density of
mycorrhiza in biennial and matrix forming perenial species of closed chalkland
turf. J. Ecology. 70: 571-593.
Giovannetti, M., and Nicolson, T.H. 1983. Vesicular arbuscular mycorrhiza in
Italian sand dunes. J. British Mycological Society. 80: 552 – 557.
Hajian Shahri, M., and Abbasi, M. 2004. Variation of Spores Vesicular–Arbuscular
Mycorrhiza Population in Pistachio Natural Forest Soil in North of Khorassan.
J. Sci. Tech. Agri.Natur. Resour. 8(4): 77-86.
Hayman, D.S. 1970. Endogone spore numbers in soil and vesicular – arbuscular
mycorrhiza in wheat as influenced by season and soil treatment. J. British
Mycological Society. 54: 53-63.
Janos, D.P. 1980. Mycorrhizae influence tropical succession. J. Biotropic 12:56-95.
Jenkins, W.R. 1964. A rapid centrifug L-technique for separating nematodes from
soil. J. Plant Disease Reporter, 48: 692.
Johnson, C.R., Menge, J.A. Chawb, S.S., and Ting, I.P. 1982. Interaction of
photoperiod and vesicular–arbuscular mycorrhiza on growth and metabolism of
sweet orange. J. New Phytologi. 90:665-669.
Johnson, N.C., Zak, D.R., Tilman, D., and Pfleger, G.L. 1991. Dynamics of
vesicular- arbuscular mycorrhizae during old field succession. J. Oecologia. 86:
349-358.
Khajehpour, M.R. 1991. Production of industrial plants. 1st edition. Academic
Center of Isfahan University of Technology. Iran. pp. 97-121.
Kianmehr, H. 1981. Vesicular arbuscular mycorrhizal spore population and
infectivity of saffron (Crocus Sativus) in Iran.J. New Phytol. 88: 79–82.
Morton, J.B. 1985. Variation in mycorrhizal and spore morphology of Glomus
occultum and Glomus diaphanum as influenced by plant host and soil
environment. J. Mycologia 77: 192-204.
Norouzi, K., Khara, J., and Ghosta, Y. 2009. Effects of three Glomus species as
biocontrol agents against verticillium-induce wilt in cotton. J. plant protection
research.49:185-189.
Olsson, P.A., Thingstrup, I., Jakobsen, L., and Bath, E. 1999. Estimation of the
biomass of arbuscular mycorrhizal fungi in a linseed field. J. Biochemical. 31:
1879- 1887.
Philips, J.M., and Hayman, D.S. 1970. Improved procedures clearing root and
staining parasitic and vesicular arbuscular mycorrhizal fungi for rapid
assessment of infections. J. British Mycological Society, 55:158- 161.
Porter,W.M., Robson, M., and Abbott, L.K. 1987. Field survey of the distribution
of vesicular – arbuscular mycorrhizal fungi in relation to soil pH. J. Applied
Soil Ecology. 24:659-662.
Rezaee Danesh, Y. 2012. Study on Status of Arbuscular Mycorrhizal Fungi
Associated with Barley in Damghan Region, Iran. J. plant protection. 26(4):
437-449.
Ross, J.P.1980. Effect of nontreated field soil on sporulation of vesicular –
arbuscular mycorrhizal fungi associated with soybean. J. Phytopathol. 70:100–
105.
Sanders, I.R. 2004. Plant and arbuscular mycorrhizal fungal diversityare we
looking at the relevant level of diversity and are we using the right techniques.J.
New Phytologyst, 164:415-418.
Schenck, N.C., Siequeira, J.O., and Oliveira, E. 1989. Changes in the incidence of
VA mycorrhizal fungi with changes in ecosystems. In V. Vancura (ed.)
Interrelationships between Microorganisms and Plant in Soil. J. Elsevier, New
York, 125-129.
Schroder, N.V. 1974. Temperature response of Endogone mycorrhiza on soybean
roots. J. Mycologia 66: 600– 605.
Shirzad, H., Panjekeh, N., and Sabbagh, S.K. 2013. Evalluation of a Vesicular
arbuscular mycorrhiza to cotton Verticillium wilt of cotton. 1st Iranian Congress
of Mycology. 3-5 Sep. University of Guilan. Iran. p.182.
Smith, S.E., Facelli, E., Pope, S., and Andrewsmith, F. 2010. Plant performance in
stressful environments: interpreting new and established knowledge of the roles
of arbuscular mycorrhizas. J. Plant Soil. 326: 3–20.
Tao, L., and Zhiwei, Z. 2005. Arbascular mycorrhizas in a hot and arid ecosystem
in southwest China. J. Applied Soil Ecology. 29: 135- 141.
Thomas, G.W. 1996. Soil pH and Soil Acidity. In Methods of Soil Analysis:
Chemical Methods. Soil Sci. Soc. of Am. 102- 108.
Trouvelot, A., Kough, J.L., and Gianinazzi-pearson, V. 1986. Mesure du taux de
mycorrhizaton VA dun systeme radiculaire. Recherché de methods destimation
ay-ant une signification functionelle. Pp. 217-221. In V. Gianinazzi-person and S.
Gia-zzi (eds.) Physiological and Genetic Aspects of Mycorrhizae. INRA, Paris.
Vestbery, M. 1995. Occurrence of some Glomales in Finland. J. Mycorrhiza, 5:
329–336.
Whipps, J.M. 2004. Prospects and limitations for mycorrhizas in biocontrol of root
pathogens. J. Canadian Botany. 82: 1198–1227.
Zak, J.C., Danielson, R.M., and Parkinson, D. 1982. Mycorrhizal fungal spore
numbers and species occurrence in two amended mine spoils in Alberta,
Canada. J. Mycology 74: 785- 792.
Zhu, Y.G., and Miller, R.M. 2003. Carbon cycling by arbuscular mycorrhizal fungi
in soil- plant systems. J. Trends in plant science. 8: 407-509.